Invited speakers | Międzyuczelniany Wydział Biotechnologii UG i GUMed

Entry of human coronaviruses

Ostatnia modyfikacja: 
piątek, 18 stycznia 2019 roku, 12:18

Speaker: Krzysztof Pyrć, PhD, DSc, JU professor (Laboratory of Virology, Jagiellonian University)
http://virogenetics.info/

Talk: Entry of human coronaviruses

Time: 25th January 2018, 9:00 am

Venue: Intercollegiate Faculty of Biotechnology, Abrahama 58, hall 042


prof. PyrćThe virus entry is one of the key points of the infection, and cell/tissue susceptibility to a large extent determines the nature and severity of the disease. Using a variety of techniques we have mapped the route of entry for several coronaviruses, including human coronavirus NL63 and human coronavirus OC43. Available data on coronavirus’ entry originate frequently from studies employing immortalized cell lines or undifferentiated cells. Here, using the most advanced 3D tissue culture system mimicking the epithelium of conductive airways, we systematically mapped entry of viruses into susceptible cell. Considering low specificity of chemical inhibitors targeting different endocytic pathways, we decided to track the fate of a single virus particle in the cell with confocal microscopy. Obtained results were validated with developed 3D image analysis algorithms and subsequent statistical assessment.

Our results show that HCoV-NL63 virions require endocytosis for successful entry, and interaction between the virus and the receptor molecule triggers recruitment of clathrin. Subsequent vesicle scission by dynamin results in virus internalization, and the newly formed vesicle passes the actin cortex, what requires cytoskeleton rearrangement. Finally, acidification of the endosomal microenvironment is required for successful fusion and release of viral genome into the cytoplasm. On the other hand, HCoV‑OC43 employed a very different route, using caveolin-1-dependent pathway to enter the cell. Surprisingly, the virus was also internalized by macropinocytosis, but this route of internalization did not allow for virus entry and subsequent replication. HCoV‑OC43 trafficking in the cell seems to be carried out along actin filaments. We believe that usage of 3D tissue culture models and an appropriate methodology allowed us to obtain reliable, relevant biological results.

 

dr Sivakumar Vadivel Gnanasundram (French Institute of Health and Medical Research)

Ostatnia modyfikacja: 
piątek, 16 listopada 2018 roku, 8:33

Speaker: dr Sivakumar Vadivel Gnanasundram (French Institute of Health and Medical Research)

Time: 16th November 2018, 9:00 am

Venue: Intercollegiate Faculty of Biotechnology, Abrahama 58, hall 042


dr gnanasundramDr. Gnanasundram finished his Master’s degree in Microbiology from the University of Madras, India in 2008. He then moved to Indian Institute of Science, India as a Research fellow and worked on novel anti-viral therapeutics using small RNAs targeting Hepatitis C virus RNA translation. For his doctoral degree he enrolled into HBIGS, Heidelberg University, Germany where he worked on Ribosome biogenesis pathway and RNA helicases. He completed his doctoral degree in 2014.  For his post-doctoral work he moved to Dr. Robin Fåhraeus group at INSERM, Paris, France.

His present work involves in understanding the oncogenic mechanisms of the Epstein-Barr virus (EBV)-encoded EBNA1. It has been known for over 50 years that EBV is a human oncogenic virus responsible for app. 2-3% of all cancers but unlike other oncogenic viruses, the underlying molecular oncogenic mechanisms of EBV have largely remained obscure. Transgenic mice models have shown a reverse correlation between the expression of the EBNA1 protein and a lymphoma phenotype. Why less of an oncogenic protein would give more cancer could not be explained until now. It turns out that EBNA1 suppresses its own synthesis in cis in order to minimize the production of antigenic peptides for the MHC class I pathway and thereby helps the virus to evade the immune system. His work that is recently published in Nature Communications shows that the stress caused by this suppression of translation leads to the activation of E2F1 and the induction of c-myc. We speculate that the virus is exploiting a cellular pathway whereby cells senses dysfunctional mRNA translation and compensates by inducing ribosomal biogenesis via c-myc.

Discovering multiple roles for AKT1 in skin barrier function and skin disease

Ostatnia modyfikacja: 
czwartek, 18 października 2018 roku, 9:12

Speaker: dr Ryan O'Shaughnessy (University of London)

Talk: Discovering multiple roles for AKT1 in skin barrier function and skin disease

Time: 19th October 2018, 9:00 am

Venue: Faculty of Chemistry, hall F8


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Dr Ryan O'Shaughnessy
is a Senior Lecturer in the Centre for Cell Biology and Cutaneous Research. He leads a research programme focused on understanding molecular mechanisms of skin barrier function and through these insights, developing new therapies for diseases of skin barrier function, with particular emphasis on the ichthyoses, eczema and skin cancer.

Ryan completed his PhD in keratinocyte biology at Cancer Research UK, before postdoctoral training at Columbia University, NY and Queen Mary University of London. He started his skin barrier laboratory in 2008 at the UCL Great Ormond Street Institute of Child Health, and in 2012 was the Academic Lead of the Livingstone Skin Research Centre, a research centre dedicated to the study of childhood skin disease. He was awarded a Society of Investigative Dermatology Kligman fellowship in 2002 and the British Society of Investigative Dermatology Young Investigator Award in 2009.

Memberships include the European Society of Dermatological Research and the European Epidermal Barrier Research Network, and the UK Translational Research Network in Dermatology (UK TREND) and he is on the Editorial Board of Experimental Dermatology and on the Committee of the British Society of Investigative Dermatology. 

Protein deubiquitylation controls the degradation of mislocalised secretory and membrane proteins and regulates endoplasmic reticulum function

Ostatnia modyfikacja: 
poniedziałek, 18 czerwca 2018 roku, 10:01

Speaker: dr Paweł Leźnicki (University of Manchester, Manchester, United Kingdom)

Talk: Protein deubiquitylation controls the degradation of mislocalised secretory and membrane proteins and regulates endoplasmic reticulum function

Time: 23th November 2018, 9:00 am

Venue: Intercollegiate Faculty of Biotechnology, Abrahama 58, hall 042


Leznicki PawelPaweł Leźnicki graduated with an MSc in Biotechnology from the Intercollegiate Faculty of Biotechnology, University of Gdańsk and Medical University of Gdańsk, Poland, in 2006. He then moved to Manchester, United Kingdom, to work on the mechanisms of post-translational delivery of tail-anchored membrane proteins to the endoplasmic reticulum. He received his PhD degree in Biochemistry in 2010. At that point his research interests shifted towards studying protein degradation mediated by the ubiquitin-proteasome system. He investigated this process and the function of deubiquitylating enzymes initially at the University of Manchester (2011-2013) and then at the MRC Protein Phosphorylation and Ubiquitylation Unit, University of Dundee, United Kingdom, a world-renowned centre for ubiquitylation-oriented research. In 2017 he moved back to Manchester to continue his work on the role of deubiquitylating enzymes in the regulation of intracellular processes.

The ubiquitin-proteasome system is the predominant degradative route for the disposal of defective and/or unwanted proteins in eukaryotic cells. Polypeptides destined for the proteasome-mediated degradation are most often initially marked by covalent conjugation of a small polypeptide, ubiquitin. Ubiquitin can form polymers (ubiquitin chains or polyubiquitin) linked via lysine residues or the N-terminus of a ubiquitin monomer, and the residue used to build such chains defines the functional outcome of protein ubiquitylation. Importantly, ubiquitylation can be reversed by the action of a group of enzymes collectively known as deubiquitylating enzymes (DUBs). By studying the quality control of membrane and secretory proteins destined to the endoplasmic reticulum (ER) we have identified several factors that control the steady-state levels of membrane and secretory proteins that fail to reach the ER. Such mislocalised proteins (MLPs) are routed towards degradation by the BAG6 complex but can be rescued by SGTA, a protein that stimulates MLP deubiquitylation. Further studies defined the molecular basis of these processes. Our subsequent work uncovered an isoform-specific function of a deubiquitylating enzyme, USP35, in apoptosis, lipid metabolism and ER stress. It also opened up new avenues for future work addressing the role of DUBs in controlling the function of intracellular organelles and pathways.

 

Unlocking the clinical utility of genomic biomarkers in ovarian cancer

Ostatnia modyfikacja: 
piątek, 11 maja 2018 roku, 13:45

Speaker: dr Anna Piskorz (University of  Cambridge)

Talk: Unlocking the clinical utility of genomic biomarkers in ovarian cancer

Time: 25th May 2018, 9:00 am

Venue: Intercollegiate Faculty of Biotechnology, Abrahama 58, hall 042


Anna Piskosz
I’m a molecular biologist focus on translational research in high grade serous ovarian cancer (HGSOC). I work on identification of genomic biomarkers that could be applied in clinic as diagnostic, prognostic and predictive biomarkers, helping in better patient stratification, earlier disease diagnosis, monitoring patient response and improving patient management during the course of treatment. My research interests focus on development and implementation of new genomic technologies with potential application in clinic like shallow whole genome sequencing for copy number alterations detection and detection of mutant tumour DNA in body fluids. In my research I use variety of specimens like tumour tissue and body fluids (blood, plasma, ascites). I have a special interest in molecular characterization of ascites samples. I’m engaged in molecular analysis of samples from several clinical trials (ARIEL2, PISSARO, ICON7, ICON8, MADCaP, PHL-093 Toronto, OZM-061 Toronto) and collaborate with scientists from other research centres: University of Calgary (Canada), NKI (The Netherlands), VU University Medical Centre (The Netherlands), Institute of Cancer Sciences University of Glasgow (UK). I'm lead consultant for the molecular analysis of HGSOC samples in collaborations with the Ovarian Tumour Tissue Analysis (OTTA) and the Multidisciplinary Ovarian Cancer Outcomes Group (MOCOG) consortiums. One of my main aims is to transform HGSOC patients' care in Addenbrooks's hospital through the implementation of real time molecular profiling of every women with HGSOC by setting up workflow between CRUK CI, pathologists, tissue bank and Cancer Molecular Diagnostic Laboratory.

How Art and Design can help Redesign Science itself; both the scientific method and the social embedding of science

Ostatnia modyfikacja: 
poniedziałek, 7 maja 2018 roku, 12:36

Speaker: prof. Roger Malina (The University of Texas at Dallas)

Talk: How Art and Design can help Redesign Science itself; both the scientific method and the social embedding of science

Time: 11th May 2018, 9:00 am

Venue: Intercollegiate Faculty of Biotechnology, Abrahama 58, hall 042


prof. MalinaRoger Malina is a physicist, astronomer and executive editor of the Leonardo publications at MIT Press. With dual appointments as a professor of arts and technology and a professor of physics at UT Dallas, he focuses on connections among the natural sciences and arts, design and humanitiesHe is a former director of the Observatoire Astronomique de Marseille Provence (OAMP) in Marseille, and member of its observational cosmology group, which performs investigations on the nature of dark matter and dark energy. He is also a member of the Mediterranean Institute for Advanced Study (Institut Méditerranéen de Recherches Avancées, IMERA), an institute he helped to organize. IMERA seeks to contribute to trans-disciplinarity between the sciences and the arts and places emphasis on the human dimensions of the sciences. Malina was also a member of the jury for the Buckminster Fuller Challenge 2011, which awards a prize to those who create strategies with potential to “solve humanity's most pressing problems.” Malina's specialty is space instrumentation. He was the principal investigator for the NASA Extreme Ultraviolet Explorer satellite at the University of California, Berkeley. The satellite was the first orbiting observatory to map the sky in the extreme ultraviolet band. The team at UC Berkeley had to invent new kinds of cameras, telescopes and data analysis techniques. The team was one of the first university groups to take over operation of a NASA satellite and operate it from a university, with teams of students. For 25 years, Malina has been involved with the Leonardo organizations, which his father founded in San Francisco and Paris. The organizations strive to promote work that explores the interactions between the arts and sciences, as well as between the arts and new technologies. Malina earned his bachelor's degree in physics from Massachusetts Institute of Technology in 1972, and his doctorate in astronomy from the University of California, Berkeley in 1979.

 
Wykład zorganizowany we współpracy MWB UG & GUMed z Centrum Sztuki Współczesnej ŁAŹNIA w Gdańsku, odbędzie się w ramach realizowanego na MWB UG & GUMed projektu STARBIOS2 (Horyzont 2020)

Inne oblicze GMO, A.D. 2018

Ostatnia modyfikacja: 
poniedziałek, 7 maja 2018 roku, 12:33

Speaker: prof. Tadeusz Twardowski (Instytut Chemii Bioorganicznej PAN w Poznaniu)

Talk: Inne oblicze GMO, A.D. 2018

Time: 27 kwietnia 2018 r., godz: 9:00

Venue: Intercollegiate Faculty of Biotechnology, Abrahama 58, hall 042


https://ug.edu.pl/media/aktualnosci/75653/prof_tomasz_twardowski_o_gmo_a...

Targeting mitochondrial DNA in lung diseases.

Ostatnia modyfikacja: 
piątek, 23 marca 2018 roku, 13:47

Speaker: prof. Bartosz Szczęsny (University of Texas Medical Branch at Galveston)

Talk: Targeting mitochondrial DNA in lung diseases

Time: 20th April 2018, 9:00 am

Venue: Intercollegiate Faculty of Biotechnology, Abrahama 58, hall 042


SzczęsnyDr. Szczesny received his BA, MS, and PhD degrees from the University of Wroclaw, Poland. He completed his post-doctoral training at the University of Texas Medical Branch at Galveston, in the United States, in Dr. Mitra’s laboratory, working on mitochondrial DNA repair. His research training includes positions at Strathclyde University, Glasgow, Scotland; Lund University, Sweden; and University in Louvain la Neuve, Belgium.

Dr. Szczesny is currently an Associate Professor in the Department of Anesthesiology at the University of Texas Medical Branch, and a Staff Scientist at the Shriners Hospital for Children in Galveston, one of the leading pediatric burn centers in the United States. Dr. Szczesny is an expert in mitochondrial DNA damage and repair, and on the signaling of mitochondrial dysfunction in various pathological conditions. His major research interests are mitochondrial dysfunction in acute lung injury and development of new treatment strategy in lung cancer targeting mitochondria/mitochondrial DNA.

 

prof. Alfredo Nicosia, University of Naples

Ostatnia modyfikacja: 
piątek, 23 marca 2018 roku, 13:48

Speaker: prof. Alfredo Nicosia, University of Naples (Chief Executive Officer (CEO) and Co-founder of Nouscom AG)

Talk: Soon

Time: 23th February 2018, 9:00 am

Venue: Intercollegiate Faculty of Biotechnology, Abrahama 58, hall 042


VG

Prof. Alfredo Nicosia is the Chief Executive Officer(CEO) and Co-founder of Nouscom AG, a biotech company dedicated to the development of next generation immunotherapies to beat cancer (www.nouscom.com). He is also CEO of Reithera srl, a Biotech involved in the development of vector-based anti-infectious vaccines.

Alfredo was a co-founder of Okairos (recently acquired by GSK) and served as the company’s Chief Scientific Officer, contributing to the preclinical and clinical development of a number of vaccines (including vaccines against HCV, Ebola and malaria) and monoclonal antibodies against infectious diseases and cancer.

Alfredo’s experience includes the positions of senior director of the Viral Diseases Vaccine program at Istituto di Ricerche di Biologia Molecolare (IRBM), staff scientist at the European Molecular Biology Laboratory (EMBL) in Heidelberg, Germany and project leader at the Sclavo Research Centre in Siena, Italy.

He has also published over 150 articles in leading journals on a range of subjects, including viral and bacterial infection and vaccines and holds a number of patents and patent applications.

Alfredo holds a degree in chemistry from the University of Rome, Italy and is a full Professor of Molecular Biology at the University Federico II, Naples (Italy).

To rot, or not to rot: comprehensive view on the interactions of phytopathogenic pectobacteria with plants

Ostatnia modyfikacja: 
piątek, 23 marca 2018 roku, 13:48

Speaker: dr Vladimir Y. Gorshkov (Kazan Institute of Biochemistry and Biophysics)

Talk: To rot, or not to rot: comprehensive view on the interactions of phytopathogenic pectobacteria with plants

Time: 9th March 2018, 9:00 am

Venue: Intercollegiate Faculty of Biotechnology, Abrahama 58, hall 042


VG

Dr. Vladimir Y. Gorshkov is a Senior Researcher at Federal Research Center: Kazan Science Centre of the Russian Academy of Sciences, Russian Academy of Science in Kazan, and a Senior Lecturer at Kazan Federal University, Russia. His main research interests are connected wiht phytopathology, plant physiology, microbiology and molecular biology. Vladimir defended his Phd thesis entitled “Cell-to-cell communication in the populations of Pectobacterium atrosepticum SCRI1043 during the interaction with host plant and under starvation” in 2009 and since then his major scientific interest is signal exchange between bacteria and host plants during interaction. Vladimir is an expert in molecular aspects of symbiosis and pathogenesis and at the moment serves as a Phd student supervisor of 3 PhD students. He is an author and co-author of more than 20 original research papers. His beloved bug is Pectobacterium atrosepticum and bellowed host plant: potato and tobacco. 

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